Sequencing

illumina HiSeq2500

DSC_9284Researchers interested in illumina sequencing services are encouraged to contact us prior to submitting samples.  During the consultation period we will address any researcher questions and discuss sample preparation, number of samples, multiplexing options, type of sequencing run, number of flow cell lanes, and data analysis.  When the experimental details are agreed upon, the core can provide the researcher with a custom cost estimate for the project if desired.

More about the HiSeq2500 . . . 

illumina NextSeq (New April 2017!)

The newest instrument in the core facility is the illumina NextSeq platform. This platform is the perfect balance between speed and throughput. With paired-end technology and supporting read lengths of up to 2×150, it is the perfect machine to run pools of RNA-Seq, ChIP-Seq and Exome-Seq data quickly and efficiently.

More about the NextSeq . . . 

illumina MiSeq

This platform specializes in more targeted sequencing geared toward smaller genomes and smaller search space investigation. This includes Amplicon, Exome and Bacterial 16S sequencing. Through our partnership with Buffalo’s Women’s Health Initiative, we will be analyzing the microbiome of several thousand Western New York women.

More about the MiSeq . . . 

Affymetrix GeneChip System

Our battle-tested Affymetrix GeneChip System is comprised of a GeneChip Scanner 3000, two Fluidics Station 450 units, and a GeneChip Hybridization Oven 640. We provide expression analysis services on all Affymetrix GeneChips including 3′ expression, miRNA, exon, andgene arrays. Initial sample quality analysis using the Agilent 2100 Bioanalyzer, target labeling, fragmentation, hybridization, staining, and scanning. Initial data analysis is conducted by the Core, while additional data analysis services can be arranged with bioinformatics specialists at the University at Buffalo.

More about the Affymetrix System . . .